Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin

Crosbi ID175268
Bib IRB ID533173
Year2011
StatusObjavljeno
Typeprilog u časopisu
Subtypeizvorni znanstveni rad
AuthorsLeščić Ašler, Ivana ; Pigac, Jasenka ; Vujaklija, Dušica ; Luić, Marija ; Štefanić, Zoran
JournalActa crystallographica. Section F, Structural biology and crystallization communications
Volume67
Issue11
Pagesstr. 1378-1381
DOI10.1107/s1744309111032222
EISSN1744-3091
Titles

en original Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin

Abstracts
en original

A recombinant lipase (triacylglycerol acylhydrolase ; EC 3.1.1.3) from the bacterium Streptomyces rimosus was inhibited by the serine protease inhibitor 3, 4-dichloroisocoumarin and crystallized by the hanging-drop vapour-diffusion method at 291 K. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 38.1, b = 78.7, c = 56.6 Å, [beta] = 104.5° and probably two molecules in the asymmetric unit. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation on the XRD beamline of the Elettra synchrotron, Trieste, Italy.

Keywords

en original streptomyces rimosus ; SGNH hydrolases ; lipases ; 3 ; 4-dichloroisocoumarin

Authors
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Citations
Leščić Ašler, Ivana ; Pigac, Jasenka ; Vujaklija, Dušica ; Luić, Marija ; Štefanić, Zoran
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin // Acta crystallographica. Section F, Structural biology and crystallization communications, 67 (2011), 11; 1378-1381. doi: 10.1107/S1744309111032222
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